HCMV-encoded miRNAs have been detected by stem-loop RTPCR. Whole RNA was isolated from HCMV-contaminated cells employing Trizol Reagent (TaKaRa) and DNA was removed making use of Recombinant DNase I (TaKaRa)
HCMV Towne pressure (ATCC-VR977) was propagated and titered as explained beforehand . For kinetic...