Ulated by the targets of miR100 and miR-125b in PDAC, we regarded the list of genes overlapping amongst transcripts loaded onto AGO2 and genes DOI: ten.1038/s41467-018-03962-x www.nature.com/naturecommunicationsNATURE COMMUNICATIONS (2018) 9:NATURE COMMUNICATIONS DOI: 10.1038/s41467-018-03962-xARTICLEbRIPaRIPmiR-125bDWmiR-DWRepresentation factorRepresentation factor4 3 two 1 0 Random Enrichment p 2.2e?4 2944 1056 244 three 2 1 0 Random Enrichment p 2.2e?cPositive z-scorez-score =Negative z-scoreNo activity pattern availableRatiod0.25 0.20 0.15 0.ten RatioPositive z-scorez-score =Negative z-scoreNo activity pattern availableRatio5 og(p -value) 4 3Threshold0.35 four og(p -value) 3Threshold0.30 0.25 Ratio 0.20 0.15 0.10 0.05 0.00 Function of CHK proteins in cell cycle checkpoint handle Function of BRCA1 in DNA harm response Molecular mechanisms of cancer Cell cycle regulation by BTG loved ones proteins ATM signaling Ceramide signaling p53 signaling ILK signaling HER-2 signaling in breast cancer Endoplasmic reticulum strain pathway Apoptosis signaling IL-6 signaling1 0 Part of CHK proteins in cell cycle checkpoint control Retinoic acid mediated apoptosis signaling Mitotic roles of polo-like kinase HIPPO signaling HER-2 signaling in breast cancer UVA-induced MAPK signaling Death receptor signaling Tight junction signaling Apoptosis signaling Ceramide signaling AMPK signaling p53 signaling0.05 0.1eP53 signalingmRNA node colour mapping ?.39 +2.5 Log2 fold changefP53 signalingmRNA node color mapping ? Log2 fold transform +MEDSTAGPIAS1 CCNG1 ADCK3 CDK4 PTENTP53IKAT2B PERPApoptosis signalingST13 CCNK PCNA FGFR1 TP53IHDACCCNK PCNATOPBP1 BBC3 SCO2 BIRC5 JMY CCND1 E2F1 RB1 CTNNB1 HIPK2 MAPKCASP6 CSNK1DHIPK2 HDAC1 E2F1 ATR PERPCDKN1A SIRTBIRC5 MDM4 HDAC9 RB1 PRKDC SFN TP53 MDM2 CCND1 GSK3B SIRT1 FAS MAPK8 TNFRSF10B THBS1 BRCA1 CTNNB1 CDKN1A JMY GNL3 EP300 BBC3 ACIN1 TP53INP1 RRM2B ATM LRDD DRAM1 JUN TP53BP2 TOPBP1 HIF1A PML MAPK14 SCO2 KAT2BApoptosis signalingTP53 MAPK8 ENDOG HTRA2 ROCK1 SPTANSERPINE2 PMAIP1 IRS2 PLAGLWT1 ROCK1 RAF1 BCL2L11 CASP9 RPS6KA1 CASP3 SPTAN1 MAP3K5 Competative Inhibitors medchemexpress DFFABADBIDJUN DRAMMED1 PRKDC GNL3 SFN PTEN HIF1A PMAIP1 EP300 ADCK3 GSK3B STAG1 MDM2 FGFR1 PIK3R3 ATR HDAC9 FAS WT1 PLAGL1 MDMBIRC6 BCL2L1 AIFM1 HTRA2 ENDOG CASP2 CYCS LMNA CASP7 BCL2ACDKBIRC6 BID CASP3 PLCG1 RAFDIABLO BCL2LCASP7 CDK1 BAKCAPNBADDFFA MAP3KATM THBS1 FRS2 CSNK1D IRS2 CASPMCL1 CASP8 PARP1 BAK1 BAX DIABLO PLCGCAPN10 CASPRPS6KA1 CYCS LMNAPARPRRM2B ST13 LRDD TP53INPMAP4KNFKB2 ACIN1 CASPAIFMPML PIAS1 SERPINE2 BCL2L11 CCNG1 Nitrification Inhibitors targets MCLBAX BCL2AmiR-125bmiR-125bCASPMARKmiR-miR-NFKBCLDN3 RAD17 PPP2R4 MDC1 PPP2R5C ATMIN RAD9A RAD50 CHEK2 MRE11A CDC25C NAPB CHEK1 CDK2 CDK1 VAPA NBN CASK OCLN LLGL1 MPP5 CDC25A HUS1 HSF1 TJP2 MYLK VASP TLK2 PRKCZ TNFRSF11B ARHGEFCNKSRPLKSTX4 CDCRFC1 CHEKF2RLBRE ATRIP BARDCLSPNCHEK1 PPP2R5D CDC25A FANCABRCAFANCE BABAM1 MSHCGN PPP2R2C CLDN12 PARD6A STX16 EPB41 MLLT4 RAC1 SYMPK SMURF1 VCL RHOA CLDN7 SAFB TJP1 MPDZRAD9A CDC25C MRE11A SLC19AMLHFANCG ATF1 BRCCRBL1 E2F6 HUS1 PLK1 PPP2R4 PPP2R5C PPP2R2C CDK2 RAD50 CLSPN MDC1 ATMIN NBN RAD17 RBBPFANCFSTAT1 UIMC1 BRCA2 BLMFANCCFANCD2 RAD51 MSHFAM175A GADD45ARole of CHK proteins in cell cycle checkpoint controlRABCTNNATight junction signalingRole of CHK proteins in cell cycle checkpoint control Edge line typeRole of BRCA1 in DNA harm responseEdge line form Seed In overlapSeedIn overlapFig. 7 miR-100 and miR-125b target similar transcripts to regulate popular pathways. a, b Venn diagrams showing miR-125b (a) or miR-100 (b) direct tar.
