Old) have been collected for 72 h. for 72 h. The image shows lipidupper lipid layers within the extraction samples. fecal samples. weeks old) have been collected The picture shows the upper the layers within the extraction tubes of fecal tubes of Quantification of (f) total fecalof (f) total fecal lipids and (g) fecal neutral sterols. (h,i) Cholesterol absorptionin chow diet-fed female mice Quantification lipids and (g) fecal neutral sterols. (h,i) Cholesterol absorption was measured was measured in chow dietfed four, 10 weeks = 4, ten a 4 h-fasting period, h-fasting gavaged with 200 gavaged with 200 corn [3 containing 2 (n =female mice (nold). Daunorubicin Cancer Afterweeks old). Immediately after a 4mice were period, mice were corn oil containing two ioilH]cholesterol i 200 cholesterol. Radioactivity was Radioactivity post-gavage in h plasma and (i) isolated tissues by liquid and [3H]cholesterol and 200 cholesterol. measured 4 h was measured four (h)post-gavage in (h) plasma and (i) isolated tissues by liquid scintillation counting. (j) Enterocyte mRNA expression of cholesterol transporters relative to Gapdh as scintillation counting. (j) Enterocyte mRNA expression of cholesterol transporters relative to Gapdh as reference gene reference gene (n = 5). Data represent 0.05 (),SD; p0.01 (), p p 0.001 (), p Student’s unpaired t-test. (n = five). Data represent signifies + SD; p implies + p 0.05 (), 0.01 (). 0.001 (). Student’s unpaired t-test.three.three. LAL-KO Intestines Accumulate Lipids from the Systemic Circulation three.3. LAL-KO Intestines Accumulate Lipids from the Systemic Circulation WTD-fed LAL-KO mice accumulate lipids predominantly inside the duodenum and WTD-fed LAL-KO mice accumulate lipids predominantly in the duodenum and jejunum, as well as the smaller intestine is markedly shorter compared to handle mice (Figure 3a). jejunum, and the modest intestine is markedly shorter in comparison with control mice (Figure 3a). We observed aa severe intestinal accumulation neutral lipids in LAL-KO micemice (Figure observed extreme intestinal accumulation of of neutral lipids in LAL-KO (Figure 3b,c). We 3b,c). Electron microscopy confirmed the of lipid-filledof lipid-filled lysosomes Electron microscopy confirmed the abundance abundance lysosomes predominantly predominantly in the (Figure 3d), which is consistent with is consistent with Velsecorat custom synthesis preceding inside the lamina propria lamina propria (Figure 3d), which preceding reports describing reports models of LAL-D [12,42,43]. We’ve got recently demonstrated the vital function of in vivo describing in vivo models of LAL-D [12,42,43]. We’ve got lately demonstrated the vital function of cytosolic lipases withinmetabolism of lipids derived in the basolateral cytosolic lipases inside enterocytes in the enterocytes inside the metabolism of lipids derived from theside from the modest intestine the tiny To figure out no matter if LAL-KO enterocytes (blood) basolateral (blood) side of [32,40]. intestine [32,40]. To identify whether or not LALKO enterocytes accumulate lipid species in the basolateral membrane of enterocytes,Cells 2021, ten,8 ofCells 2021, 10, x8 ofaccumulate lipid species in the basolateral membrane of enterocytes, we incorporated we incorporated [3H]oleate into an intralipid emulsion, injected it intraperitoneally, and [3 H]oleate into an intralipid emulsion, injected it intraperitoneally, and measured the tracer 3 measured the tracer in diverse intestinal segments [32]. [3 H]oleate as an alternative of cholesterol in different intestinal segments [32]. The incorporation from the incorporation of [.
