ACE2 in enterocytes), SLC7A9 (which codes for an L-DOPA influx transporter) and SLC16A10 (which codes for an L-DOPA efflux transporter). In the entire set of data (n = six, two handle samples, two samples at 24 h post-infection and two samples at 60 h post infection), we could extract expression values for 11 out of 14 genes of interest. We then utilized the Pearson’s correlation test to evaluate the co-expression hyperlinks between these genes and ACE2. We found that eight key genes involved in the metabolism of dopamine and/or trace amines exhibited statistically considerable co-expression hyperlinks with ACE2 across all experimental conditions. Of note, the most robust correlation link was observed for MAOB, followed by SLC7A9 and SULT1A1 (Table three).Int. J. Mol. Sci. 2021, 22,tern. Furthermore anticipated, the L-DOPA efflux transporters SLC3A2 and SLC7A8 were detected in the basolateral membrane of enterocytes. A low and diffuse staining pattern was observed for SLC16A10. Ultimately, no TH staining might be detected (Figure S1), in accordance with genomics analyses. Depending on these mined data, a scheme summarizing the ALK1 Purity & Documentation predicted dopamine/trace amines metabolic pathways taking spot in human enterocytes six of 16 is shown in Figure two.Figure two. Functional scheme summarizing the predicted dopamine/trace amines metabolic pathways taking spot in human Figure two. Functional scheme summarizing the predicted dopamine/trace amines metabolic pathways taking location in huenterocytes of of modest intestine. This scheme is based on the mining of human expression atlases and on previously man enterocytesthe the smaller intestine. This scheme is based onthe mining of human expression atlases and on previously publishedbiochemical and/or functional information obtained in intestinal or non-intestinal cells. The molecules incorporated within this published biochemical and/or functional information obtained in intestinal or non-intestinal cells. The molecules included within this scheme comprise: angiotensin-converting enzyme (ACE2), solute carrier family members 6 member 19 (SLC6A19), solute carrier scheme comprise: angiotensin-converting enzyme two two (ACE2), solute carrier family six member 19 (SLC6A19), solute carrier household 33member 11(SLC3A1), solute carrier family 77member 99(SLC7A9), dopa-decarboxylase (DDC), sulfotransferase family member (SLC3A1), solute carrier household member (SLC7A9), dopa-decarboxylase (DDC), sulfotransferase household 1A member 11 (SULT1A1),sulfotransferase loved ones 1A member 22 (SULT1A2),sulfotransferase family members 1A member 33 household 1A member (SULT1A1), sulfotransferase family members 1A member (SULT1A2), sulfotransferase loved ones 1A member (SULT1A3), cytochrome P450 household two subfamily D member six (CYP2D6), monoamine CYP1 review oxidase A (MAOA), monoamine oxidase B (MAOB), solute carrier loved ones three member 2 (SLC3A2), solute carrier household 7 member eight (SLC7A8) and solute carrier household 6 member 10 (SLC16A10). Table 3. Correlation evaluation of ACE2 mRNA levels with important genes in the dopamine/trace amines metabolic pathways in SARS-CoV2-infected human enterocytes. DDC 0.84 0.035 MAOA 0.86 0.025 MAOB 0.96 0.001 SULT1A1 0.92 0.007 SLC7A9 0.95 0.003 SLC3A1 0.87 0.02 SLC6A19 0.88 0.017 SLC3A2 0.9 0.Expression data had been extracted from Lamers et al. [34] along with the Pearson’s test was applied to assess correlation coefficient (r, upper line) and statistical significance (p-value, decrease line)) in between ACE2 and genes of interest. Gene symbols: dopa-decarboxylase (DDC), monoamine oxidase A (MAOA), monoamine oxidase B (MAOB), solute carr
