tively correlated genes (29.41 ) were Caspase 10 Activator Purity & Documentation enriched in 11 pathways, with four CB2 Agonist supplier pathways being substantially enriched, namely RNA transport, ascorbate and aldarate metabolism, fatty acid biosynthesis, and porphyrin and chlorophyll metabolism. In HSK0/ HSK48, 13 negatively correlated genes (26.53 ) had been enriched in 23 pathways, with 5 pathways becoming drastically enriched, namely ubiquinone and other terpenoid-quinone biosynthesis, sulfur relay program, thiamine metabolism, selenocompound metabolism, and ether lipid metabolism. In HSK0/HRK0, 56 negatively correlated genes (39.72 ) had been enriched in 65 pathways, with six pathways being drastically enriched, namely pyruvate metabolism, propanoate metabolism, glyoxylate and dicarboxylate metabolism, carbon metabolism, lipoic acid metabolism, carotenoid biosynthesis. In HSK48/ HRK48, 72 negatively correlated genes (62.07 ) had been enriched in 58 pathways, with five pathways being substantially enriched, namely carbon metabolism, propanoate metabolism, lipoic acid metabolism, pyruvate metabolism, biosynthesis of amino acids. The outcomes recommended that a variety of defense responses would be activated when soybean had been subjected to bean pyralid larvae tension. Additionally, KEGG enrichment analysis of negatively correlated genes positioned in the promoter regions showed that (Table S3), in HRK0/HRK48, two negatively correlated genes (18.18 ) were enriched in four pathways. In HSK0/HSK48, five negatively correlated genes (50.00 ) have been enriched in 10 pathways, with 2 pathways becoming significantly enriched, which includes monoterpenoid biosynthesis, and SNARE interactions in vesicular transport. In HSK0/HRK0, 30 negatively correlated genes (30.61 ) were enriched in 42 pathways, with five pathways becoming significantly enriched, such as endocytosis, glycolysis/ gluconeogenesis, pyruvate metabolism, RNA degradation, sesquiterpenoid and triterpenoid biosynthesis. In HSK48/HRK48, 31 negatively correlated genes (36.90 ) had been enriched in 40 pathways, with 3 pathways being substantially enriched, which includes endocytosis, monoterpenoid biosynthesis, sesquiterpenoid and triterpenoid biosynthesis.Zeng et al. BMC Genomics(2021) 22:Page 8 ofFunctional evaluation of your negatively correlated genesFor further understanding the resistance mechanism on the soybean to bean pyralid larvae, Mercator application was employed to receive the classification statistics of 265 negatively correlated genes, of which 114 had been annotated into 24 categories (Table S4). These genes have been determined to be mostly related to such pathways as protein biosynthesis and modifications; main metabolism; secondary metabolism; cell cycle, cell structure and component; phytohormone action; external stimuli responses, and so on. A total of 31 DEGs associated to protein metabolism and modification (Table S4), among them, two genes have been upregulated in HRK0/HRK48; five genes had been up-regulated and 5 genes have been down-regulated in HSK0/HSK48; 3 genes were up-regulated and a single gene was downregulated in HSK0/HRK0; a single gene was up-regulated and 3 genes have been down-regulated in HSK48/HRK48; six genes were up-regulated and three genes have been downregulated in HSK0/HRK0 and HSK48/HRK48; one particular gene was up-regulated in HRK0/HRK48 and HSK0/HSK48. 27 DEGs associated to cell cycle, cell structure, and cell element, of which 5 genes were up-regulated in HRK0/ HRK48; seven genes have been up-regulated in HSK0/HSK48; nine genes had been up-regulated and two genes had been downregulated in HSK0/HRK0; two genes had been up-regul
