Impact of Res Res on the Expression of Phase-I Metabolic Enzyme CYP450 in AFB1 As shown in Figure four, compared with the control group, the mRNA relative expression As shown in Figure 4, compared using the manage group, the mRNA relative expreslevels of CYP1A1, CYP1A4 and TLR3 Accession CYP3A4 genes (Figure 4A) and protein expression levels of sion levels of CYP1A1, CYP1A4 and CYP3A4 genes (Figure 4A) and protein expression CYP1A1 and CYP3A4 (Figure 4B) inside the liver were significantly elevated (p 0.05) within the levels of CYP1A1 and CYP3A4 (Figure 4B) within the liver have been considerably improved (p 0.05) in the AFB1 group. The supplementation of Res within the ducks’ diets considerably decreased the mRNA relative expression of the CYP3A4 gene and protein expression levels of CYP1A1 and CYP3A4 (p 0.05) compared with all the AFB1 group.Animals 2021, 11,9 ofAnimals 2021, 11, x FOR PEER Critique mRNAAFB1 group. The supplementation of Res inside the ducks’ diets drastically decreased the ten of 19 relative expression on the CYP3A4 gene and protein expression levels of CYP1A1 and CYP3A4 (p 0.05) compared using the AFB1 group.Figure four. Expression of phase I metabolizing enzyme in the duck liver exposed to AFB1. (A): mRNA levels in the associated genes of phase- I metabolic enzymes. (B): protein levels with the connected genes of Figure four. Expression of phase I metabolizing enzyme inside the duck liver exposed to AFB1. (A): mRNA phase- I metabolic enzymes. Values are represented as the mean SEM (n = 6). a Mean values with levels with the connected genes of phase- I metabolic enzymes. (B): protein levels of the connected genes of very same superscript letters or no letters within a row were of no NLRP3 list considerable distinction (p 0.05), these phase- I metabolic enzymes. Values are represented as the imply SEM (n = 6). a Mean values with with distinctive superscriptor no letters inside a row have been of no considerable distinction (p 0.05), those very same superscript letters letters have been of important or extremely significant difference (p 0.05).three.6. Effect of Res on GSH Content and mRNA Expression of Associated Regulatory Genes in Liver of AFB1-Exposed Duck three.six. Effect of Res on GSH Content and mRNA Expression of Associated Regulatory Genes in Liver GSH is often a cofactor of AFB1-Exposed Duck that mediates the detoxification of GST and is conducive for the metabolism of toxic substances in the liver. GSH synthesis is regulated by GCLC and GCLM in the GSH is usually a shown inthat mediates the detoxification ofdifference in the mRNA towards the liver. As cofactor Figure five, there was no substantial GST and is conducive levels metabolism of toxic substances inside the liver. GSH synthesis is regulated by GCLC and of the GCLM gene in livers among the control group, the AFB1 group and also the AFB1 + Res GCLM inside the liver. As shown in Figure five, there was no important distinction inside the mRNA group. Compared together with the control group, AFB1 exposure significantly decreased GSH levels contentof the 0.05), GST activity (p 0.01), and mRNA levels AFB1 group and (p AFB1 (p GCLM gene in livers among the control group, the of genes (GST) the 0.05) + Res group. Compared with all the handle group, AFB1 exposure substantially decreased within the liver in the AFB1 group. Compared with the AFB1 group, the GSH content, GST GSH content (p 0.05), GST activity and the mRNA levelsactivity (p 0.01), and mRNA levels of genes increasedin the of GST and GCLC genes had been considerably (GST) (p 0.05) inside the liver in the AFB1 group. Compared with all the AFB1 group, the GSH content material, G
