Ation of each on systolic blood stress throughout the experiment (A). Effects of ALSK and L-arg remedy in renovascular hypertension on the concentration-response curves to PPARβ/δ Antagonist web phenylephrine (B), acetylcholine (C) and sodium nitroprusside (SNP) (D) in the aortic rings. Data are reported as indicates E. The amount of animals in each and every group is indicated in parentheses. P,0.05 vs Sham; #P,0.05 vs ALSK; {P,0.05 + vs L-arg; +P,0.05 vs ALSK+L-arg (two-way ANOVA, followed by Tukey’s post hoc test).all analyses, the differences were considered significant at P,0.05.ResultsEffect of ALSK and L-arginine treatment on SBP The baseline SBP (time 0) was similar in the 5 experimental groups before surgery (Sham: 112.21.01 mmHg, n=7; 2K1C: 120.4.11 mmHg, n=7; ALSK: 124.6.20 mmHg, n=8; L-arg: 115.63.3 mmHg, n=8, and ALSK+L-arg: 118.82.70 mmHg, n=8), and no significant change in SBP was seen in the Sham group at the end of treatment (114.4.2 mmHg, n=7). Surgical renal stenosis was associated with a significant increase in SBP compared with the sham operation, and was detectable as early asTable 1. Parameters of maximal response (Rmax) and sensitivity (pD2) of the concentration-response curves to phenylephrine in the aortas from all experimental groups, before (E+) and after endothelial denudation (E and after incubation with L-NAME (100 mM), + losartan (10 mM), apocynin (0.3 nM) and SOD (150 U/mL). + Control (E+) Sham Rmax pD2 2K1C Rmax pD2 ALSK Rmax pD2 L-arg Rmax pD2 ALSK + L-arg Rmax pD2 92.4 4.41 6.77 0.35 148.1 15.6 7.25 0.14 112.3 7.4 7.74 0.21 106.6 8.81 8.20 0.24 84.39 7.61 8.28 0.371 E130.3 3.66H 7.93 0.20H 166.4 7.59 8.90 0.32H 136.7 12.9 8.23 0.20 161.8 10.5H 8.60 0.20 162.4 13.9H 8.13 0.43 L-NAME 133.5 10.6H 8.45 0.30H 163.1 8.7 7.94 0.12H 218.5 40.7H 7.84 0.28 158.1 9.1H 8.06 0.26 187.8 19.1H 8.37 0.15 Losartan Apocynin SOD93.3 6.8 7.55 0.28 86.9 8.6H 7.93 0.32 78.8 6.4H 8.47 0.15 44.7 9.01H 10.9 2.87 52.8 6.31H 7.7 0.91.7 3.6 7.83 0.21 42.7 4.32H 7.70 0.17 60.7 16.8H 10.2 1.85 85.2 17.1 14.4 3.05 35.7 5.0{H 10.5 1.87.4 6.8 7.3 0.21 62.9 7.1H 7.8 0.25H 72.9 8.3H 7.7 0.31 72.2 14.1 8.3 0.34 67.3 4.7 8.5 0.Data are reported as means E. Rmax: maximal effect (reported as a percentage of the maximal response induced by 75 mM KCl); pD2: og one-half Rmax; E endothelium removal; L-NAME: NG-nitro-L-arginine methyl ester; SOD: superoxide dismutase. P,0.05 + vs Sham; 1 P,0.05 vs 2K1C; { P,0.05 vs L-arg; and H P,0.05 vs control E+ (two-way ANOVA, followed by Tukey’s post hoc test).Braz J Med Biol Res 48(1)bjournal.MMP-9 Activator Storage & Stability brAliskiren+L-arginine prevents endothelial dysfunction +Figure 2. Effects of endothelium removal (E on the concentration-response curve for phenylephrine in the aortic rings from Sham (A), + + 2K1C (B), aliskiren (ALSK) (C), L-arginine (L-arg) (D) and ALSK+L-arg (E) treatment in the aortic rings with (E+) and without (E endothelium. The differences in the area under the concentration-response curves (dAUC) in endothelium-denuded and intact segments is shown in F. Data are reported as means E. The number of animals in each group is indicated in parentheses. #P,0.05 + vs ALSK; 1P,0.05 vs 2K1C and HP,0.05 vs E+ (two-way ANOVA, followed by Tukey’s post hoc test).days after surgery (2K1C: 2042.7 mmHg, n=7; ALSK: 217.80.2 mmHg, n=7; L-arg: 197.5.9 mmHg, n=8; ALSK+L-arg: 197.1.08 mmHg, n=8 vs Sham: + 119.2.51). After 21 days of treatment, only the combined administration of ALSK+ L-arg (138.4+ 4.37 mmHg, n=8) was effective in reducing SBP (P,0.05) comp.
