N liver homogenate. J. Biol. Chem. 1957; 224:46375. [PubMed: 13398422] 32. Mayer FV, Heath R, Underwood E, Sanders MJ, Carmena D, McCartney RR, Leiper FC, Xiao B, Jing C, Walker PA, Haire LF, Ogrodowicz R, Martin SR, Schmidt MC, Gamblin SJ, Carling D. ADP regulates SNF1, the Saccharomyces cerevisiae homolog of AMP-activated protein HDAC6 Inhibitor Compound kinase. Cell Metab. 2011; 14:70714. [PubMed: 22019086] 33. Rubenstein EM, McCartney RR, Zhang C, Shokat KM, Shirra MK, Arndt KM, Schmidt MC. Access denied: Snf1 activation loop phosphorylation is controlled by availability with the phosphorylated threonine 210 towards the PP1 phosphatase. J. Biol. Chem. 2008; 283:22230. [PubMed: 17991748] 34. Nigavekar SS, Tan YS, Cannon JF. Glc8 can be a glucose-repressible activator of Glc7 protein phosphatase-1. Arch. Biochem. Biophys. 2002; 404:719. [PubMed: 12127071] 35. Castermans D, Somers I, Kriel J, Louwet W, Wera S, Versele M, Janssens V, Thevelein JM. Glucose-induced posttranslational activation of protein phosphatases PP2A and PP1 in yeast. Cell Res. 2012; 22:1058077. [PubMed: 22290422] 36. Isom DG, Sridharan V, Baker R, Clement ST, Smalley DM, Dohlman HG. Protons as second messenger regulators of G protein signaling. Mol. Cell. 2013; 51:53138. [PubMed: 23954348] 37. Inoki K, Kim J, Guan KL. AMPK and mTOR in cellular power homeostasis and drug targets. Annu. Rev. Pharmacol. Toxicol. 2012; 52:38100. [PubMed: 22017684] 38. Shoichet BK, Kobilka BK. Structure-based drug screening for G-protein-coupled receptors. Trends Pharmacol. Sci. 2012; 33:26872. [PubMed: 22503476]NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptSci Signal. Author manuscript; offered in PMC 2014 July 23.Clement et al.Page39. Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, Maekawa H, Moreno-Borchart A, Doenges G, Schwob E, Schiebel E, Knop MA. versatile toolbox for PCR-based tagging of yeast genes: New fluorescent proteins, a lot more markers and promoter substitution cassettes. Yeast. 2004; 11:94762. [PubMed: 15334558] 40. Brachmann CB, Davies A, Cost GJ, Caputo E, Li J, Hieter P, Boeke PD. Designer deletion strains derived from Saccharomyces cerevisiae S288C: A helpful set of strains and plasmids for PCRmediated gene disruption and also other applications. Yeast. 1998; 14:11532. [PubMed: 9483801] 41. Stols L, Gu M, Dieckman L, Raffen R, Collart FR, Donnelly MI. A new vector for highthroughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage website. Protein Expr. Purif. 2002; 25:85. [PubMed: 12071693] 42. Lee MJ, Dohlman HG. Coactivation of G protein signaling by cell-surface receptors and an intracellular exchange element. Curr. Biol. 2008; 18:21115. [PubMed: 18261907] 43. Dohlman HG, Goldsmith P, Spiegel AM, Thorner J. Pheromone action regulates G-protein subunit myristoylation in yeast Saccharomyces cerevisiae . Proc. Natl. Acad. Sci. U.S.A. 1993; 90:9688692. [PubMed: IDO Inhibitor Purity & Documentation 8415763] 44. Heenan EJ, Vanhooke JL, Temple BR, Betts L, Sondek JE, Dohlman HG. Structure and function of Vps15 within the endosomal G protein signaling pathway. Biochemistry. 2009; 48:6390401. [PubMed: 19445518] 45. Studier FW. Protein production by auto-induction in higher density shaking cultures. Protein Expr. Purif. 2005; 41:20734. [PubMed: 15915565] 46. Torres MP, Lee MJ, Ding F, Purbeck C, Kuhlman B, Dokholyan NV, Dohlman HG. G protein mono-ubiquitination by the Rsp5 ubiquitin ligase. J. Biol. Chem. 2009; 284:8940950. [PubMed: 19176477] 47. Hoffman GA, Garrison TR, Dohlman HG. Analysis of RGS p.
