Ntification of those microorganisms [24]. The 18 selected strains represented, altogether, the six rep-PCR clusters. All strains yielded single amplification items of the anticipated size (about 1,500 bp) for the 16S rRNA genes and showed H1 Receptor Agonist supplier identical restriction RsaI profiles (data not shown), characteristic from the genus Azotobacter [2, 24]. When ARDRA was performed utilizing HhaI, six different profiles had been obtained. Cluster analysis of HhaI restriction profiles revealed 4 distinct clusters at 80 similarity level (Figure 2). Because all strains grouped incluster I showed profiles distinctive of A. chroococcum, as reported by Aquilanti et al. 2004 [2], and identical to those of A. chroococcum reference strain BNM 272, they had been assigned to this species. Cluster II included only strain AT33, which showed a characteristic banding profile of the species A. armeniacus [2], whereas cluster III contained only the three A. DYRK4 Inhibitor Purity & Documentation vinelandii strains utilised as reference. The ARDRA profiles of strains in cluster IV, obtained experimentally, were comparable to those of A. salinestris reference strains ATCC 49674T and I-A accomplished in silico. Based on these final results, the strains of heterogeneous cluster IV (Figure two) were assigned to A. salinestris. To confirm species identification of isolates, partial sequencing of your 16S rRNA gene was performed for seven strains representing ARDRA clusters. Based on the similarity observed amongst these sequences, strains AT25 and AT31 in cluster I (Figure 2) had been related to A. chroococcum LMG 8756T (99 identity), strain AT33 in cluster II was relatedTable 1: Geographical origin and land use of soil samples from which Azotobacter isolates were obtained. Summary of fingerprinting and identification results of isolates and soil chemical traits. Isolate OM ( ) 3.38 five.72 1.86 1.05 0.98 8.00 eight.45 eight.20 five.80 1.21 0.43 1.45 0.48 7.30 0.48 AT25 AT22 AT30 AT31 AT4 AT5 AT9 AT24 AT28 AT43 1 I 1 I 1 nd A. chroococcum A. chroococcum A. chroococcum 1 I A. chroococcum 1 nd A. chroococcum 5.72 2.74 3.15 1.02 0.19 0.17 A. chroococcum A. armeniacus HQ623182 four.47 three.12 7.83 six.40 8.30 6.60 eight.77 eight.60 7.00 6.13 0.80 0.49 0.66 1.58 0.28 0.20 0.93 0.69 eight.50 40.40 45.80 8.ten four.80 4.50 13.50 ten.ten 1 I A. chroococcum 1 I A. chroococcum HQ623181 1 I A. chroococcum 1 nd A. chroococcum 1 I A. chroococcum HQ623180 rep-PCR group ARDRA cluster Species assignments Partial 16S rDNA sequence (accession number) Soil chemical parameters pH EC (mS cm-1 ) P (ppm) 7.40 51.00 1.90 7.70 eight.Sampling siteMaize stubbleThe Scientific World JournalAgricultural bareLagoon bankLagoon bankGeographical origin Buenos Aires (Azul) Buenos Aires (Balcarce) Buenos Aires (Mar Chiquita) Buenos Aires (Mar Chiquita) Buenos Aires (Santa Clara del Mar)Urban landSide of roadNatural pastureNatural pastureRiver bankBuenos Aires (Santa Clara del Mar) Chubut (Esquel) Chubut (Gaiman) Chubut (Trevel ) i Jujuy (Tilcara) AT11 AT13 AT26 AT27 AT39 AT32 II 2 nd 1 nd 1 1 1 1 nd nd nd I A. chroococcum A. chroococcum A. chroococcum A. chroococcumSide of routeaJujuy (Tilcara)Natural pastureWheat cropWheat cropWheat crop two nd IV IV ndATA. armeniacus3.6.0.11.Wheat crop 3 AT19 AT14 AT1 3ATA. armeniacus HQ591467 A. salinestris A. salinestris A. salinestris3.15 1.78 1.64 3.six.06 six.40 7.80 7.0.52 0.21 2.24 nd11.ten 48.80 3.30 127.Soybean cropNatural pastureEntre R s i (Paran? a C?rdoba o (Corral de Bustos) C?rdoba o (Corral de Bustos) C?rdoba o (Corral de Bustos) Salta (Embarcaci?n) o Salta (Joaqu V. i Gonz e.
