Acid; CPS, copalyl pyrophosphate synthase; KS, ent-kaurene synthase; GA20ox, GA-20 oxidase; GID1, GA-insensitive dwarf 1; 4CL, 4-coumarate CoA ligase; CCR, cinnamoyl CoA reductase; CAD, cinnamyl alcohol dehydrogenase; CCaOMT, caffeoyl CoA O-methyltransferase; SCP, serine carboxypeptidaseSu et al. BMC Genomics (2016) 17:Page 19 ofactive in Yacheng05-179, may possibly contribute to smut resistance in sugarcane. The calcium signaling, ROS/NO and ABA pathways, which have been repressed by S. scitamineum, may possibly not be critical for smut resistance in sugarcane. These findings shed new light on the differential expression of proteins in sugarcane in response to S. scitamineum infection.RT-qPCR: Reverse transcription quantitative real-time polymerase chain reaction; SAR: Systemic acquired resistance; SCP: Serine carboxypeptidase; SCX: Strong cation exchange; SE: Standard error; SnRK2: Sucrose nonfermenting-1-related protein kinase two; TCA: Trichloroacetic acid; TEAB: Tetraethyl-ammonium bromide; TMV: Tobacco mosaic virus Acknowledgements The authors give specific due to Mingjie Li (Fujian Agriculture and Forestry University, Fuzhou, China) at the same time because the Beijing Genomics Institute (BGI, Shenzhen, China) for helpful discussions on protein identification and quantification analysis.GRO-alpha/CXCL1 Protein Formulation Funding This function was supported by Organic Science Foundation of Fujian province, China (2015J06006 and 2015J05055), the Analysis Funds for Distinguished Young Scientists in Fujian Agriculture and Forestry University (xjq201630), the Program for New Century Superb Talents in Fujian Province University (JA14095), the earmarked fund for the Contemporary Agriculture Technologies of China (CARS-20) and Analysis Funds for Distinguished Young Scientists in Fujian Provincial Department of Education (JA13090).Protease Inhibitor Cocktail web Availability of information and materials The information supporting the conclusions of this article are within the paper and its more files. Authors’ contributions YCS, LPX and YXQ conceived, created and initiated the project. YCS and ZQW ready materials. YCS, ZQW, QP, YTY and YC performed experiments and contributed to information analysis and validation. YCS drafted the manuscript.PMID:23453497 LPX and YXQ helped to revise the manuscript. All authors study and authorized the final manuscript. Competing interests The authors declare that they have no competing interests. Consent for publication Not applicable. Ethics approval and consent to participate Not applicable. Author details 1 Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China. two Guangxi Collaborative Innovation Center of Sugarcane Business, Guangxi University, Nanning 530005, China. Received: 13 January 2016 Accepted: five OctoberAdditional filesAdditional file 1: Text S1. Information from the transitions choice and MRM strategy validation. (DOCX 16 kb) More file 2: Table S1. The primers utilised for RT-qPCR amplification of correlated differentially expressed genes. (DOCX 23 kb) Further file 3: Figure S1. The detection of gDNA PCR and total RNA RT-PCR of resistance plants for ScGluA1 gene. M, DNA marker 15,000 + 2000 bp; 1 five, The gDNA PCR amplification solutions of transgenic plants; 1′ 5′, The total RNA RT-PCR amplification products of transgenic plants; 6 and 6′, The amplification merchandise of pCAMBIA 1301-ScGluA1; 7 and 7′, The amplification goods of non-transgenic plants; eight and 8′, Blank handle. (DOCX 242 kb) More file 4: Table S2. List of th.
