Pointed some pathways of distinct interest (see Table 1). Among up-regulated genes, by far the most substantial KEGG pathways are associated to muscle development/contraction and glucose metabolism, even though the Hedgehog signalling pathway (dre04340) and Wnt signalling pathway (dre04310) are amongst one of the most important pathways represented by genes down-regulated more than time. Essential elements of muscle improvement and function such as caveolin three (N_isotig07042), troponin T (N_isotig13004), tropomyosin (P_isotig00564) and cholinergic receptor, nicotinic, alpha 1 (CHNRA, N_isotig07602), which modulate muscle contraction at the same time as numerous kind of myosin, show a rise in gene expression more than time (Table 1, an heatmat showing gene expression values is reported in Additional file two). Glucose metabolism, specifically glycolysis, is represented by quite a few genes displaying the identical trend; aldolase a (P_contig00403), glucose phosphate isomerase b (N_isotig03674), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, N_isotig18841), lactate dehydrogenase (P_isotig00860) and a lot of other folks improve in expression much more than 20-fold from 1 to 33 dph. By contrast, genes integrated within the pathways “Hedgehog signalling” and “Wnt signalling” displayed decreasing expression over time (see Table 1, Further file two for corresponding heatmaps). These two important pathways are involved in developmental processes and control of asymmetric cell division. In specific, a big variety of genes related to “Hedgehog signalling” displayed a decreasing temporal trend of expression, for example sonic hedgehog-like (P_isotig18139), bone morphogenetic protein 7b (P_isotig17732), megalin (P_isotig07996), and hedgehog interacting protein (N_isotig19239). Likewise,N_isotig04075 N_isotig11618 N_isotig08672 N_isotig19778 N_isotig05817 P_isotig17511 N_isotig01855 N_isotig03727 P_isotig01165 P_isotig18441 N_isotig17357 Probe Name N_contig01740 N_isotig13675 N_isotig03674 P_isotig13251 P_contig00403 N_isotig03547 P_isotig10593 P_isotig18039 N_isotig03683 N_isotig04393 N_isotig06502 N_isotigGLUCOSE METABOLISMFerraresso et al. BMC Genomics 2013, 14:315 http://www.biomedcentral/1471-2164/14/Page 5 ofTable 1 Genes up- or down-regulated more than time (Continued)N_isotig18841 P_isotig00860 P_isotig00400 N_contig00167 N_isotig05258 N_isotig12521 N_isotig06792 P_isotig13923 P_isotig03551 N_isotig20169 ENSDARG00000039914 ENSDARG00000040856 ENSDARG00000043180 ENSDARG00000054191 ENSDARG00000057571 ENSDARG00000057630 ENSDARG00000060797 ENSDARG00000062998 ENSDARG00000070826 ENSDARG00000071076 Glyceraldehyde-3phosphate dehydrogenase Lactate dehydrogenase A4 Glycerol-3-phosphate dehydrogenase 1b Phosphoglycerate kinase 1 Phosphoglycerate mutase two (muscle) Aldose 1-epimerase Phosphofructokinase, muscle b Peptidoglycan recognition protein two 2,3-bisphosphoglycerate mutase Related to L-lactate dehydrogenase B chain Gene name GLI-Kruppel family members member GLI3 Casein kinase 1, delta a Casein kinase 1, gamma 2a Glycogen synthase kinase three beta (GSK3B) Casein kinase 1, gamma 2b Bone morphogenetic protein 5 Wingless-type MMTV integration website household,7Bb (WNT7) Hedgehog interacting protein (HiP) F-box and WD-40 domain protein 11b (FBXW11) Equivalent to cAMP-dependent protein kinase (PKA Calpha) Protein kinase, cAMPdependent, catalytic, beta Megalin, low density lipoprotein-related protein two (LRP2) Casein kinase 1, alpha 1 Zic loved ones member two Bone morphogenetic protein 7b Sonic hedgehog-like; Sonic hedgehog aTable 1 Genes up- or down-reg.Chloroquine phosphate Ofloxacin PMID:23357584
