Product Name :
TOP10 F＇
Applications:
phage display system
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Description:
TOP10F´ E. coli are identical to TOP10 cells, with the addition of an F´ episome. The F´ episome carries the tetracycline resistance gene and allows isolation of single-stranded DNA from vectors that have an f1 origin of replication.{{2648058-48-2} medchemexpress|{2648058-48-2} Protocol|{2648058-48-2} In Vitro|{2648058-48-2} manufacturer} In addition, the F´ episome carries the lacIq repressor for inducible expression from trc, tac, and lac promoters using IPTG.{{139290-65-6} site|{139290-65-6} Technical Information|{139290-65-6} Description|{139290-65-6} manufacturer} TOP10F´ cells require IPTG induction for blue/white color screening.PMID:30252350 TOP10F´ cells are lacIq and require IPTG to induce expression from the lac promoter. If blue/white screening is required to select for transformants, spread 40 µl of 40 mg/ml X-Gal in dimethylformamide and 40 µl of 100 mM IPTG on top of the agar. Let the X-Gal and IPTG diffuse into the agar for approximately 1 hour.IntroductionStrains Resistance tetracycline resistanceCulture Medium LBCondition 37℃ ,under the aerobic conditionsGenotypeF'[lacIqTn10(tetR)] mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1nupG
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