Rabbit anti-N-cadherin Polyclonal Antibody

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Product Details FAQ Citations(1) Video Pictures Documents |Overview |Synonym Cadherin 2; Cadherin 2 N cadherin neuronal; Cadherin 2 type 1; Cadherin 2 type 1 N cadherin neuronal; cadherin 2 type 1 N-cadherin neuronal; Cadherin2; Calcium dependent adhesion protein neuronal; CD325; CD325 antigen; CDH2; CDHN; CDw325; CDw325 antigen; N cadherin 1; NCAD; Neural Cadherin |Host Rabbit |Specificity N-cadherin |Species Reactivity Human, Mouse, Cow |Predicted Reactivity Rat, Pig, Danio reri |Applications WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |Immunogen KLH conjugated synthetic peptide derived from human N-cadherin:701-800/905 |Properties |Concentration 1mg/ml |Purification affinity purified by Protein A |Clonality Polyclonal Antibody |Isotype IgG |Storage Temp. Store at -20 ° C for one yearAvoid repeated freeze/that cycles |Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |Research areas Tumor Cell biology Immunology Kinases and phosphatases Cell adhesion molecules |Target |Background This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. |Cellular localization Cell membrane; |UniProt P19022 | Sample: HepG2 Cell Lysate at 40 ug Eye (Mouse) Lysate at 40 ug Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- N-cadherin (abs121341)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 100kDObserved band size: 110kD| Sample: Colon carcinoma(Human) lysate at 30ug; Brain(Rat) lysate at 30ug; Primary: Anti-CDH2/N-cadherin (abs121341) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG at 1: 3000 dilution; Predicted band size : 100kDObserved band size : 100kD| Sample: Breast ca (Mouse) Lysate at 40 ugPrimary: Anti-N-cadherin (abs121341) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 100 kDObserved band size: 105 kD| Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-N-cadherin Polyclonal Antibody, Unconjugated secondary primary antibody 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining| Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-N-cadherin Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining| Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-N-cadherin Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugatedused at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei| Blank control: Hepg2(blue). Primary Antibody:Rabbit Anti-E cadherin antibody; Dilution: 3μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.ProtocolThe cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (1 hour) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. Acquisition of 20,000 events was performed. |Tips:This product is for research use only. Not for use in diagnostic prodcedures.