And trough of each wave in a dataset and subtracting the mean maximum from the mean minimum). Two different datasets were analyzed in these measurements–one from a healthy heart and one from an erratically beating heart (Figure S1). The results 
demonstrate that in both cases, the segmentation approach based on frequency- and time-domain analysis better predicts manual measurements. While the methods yield different absolute values of SV, their ability to detect changes in these parameters is nearly identical (figure 5 and 5c). These methods of analysis therefore verify one another in their ability to detect the effects of cardiotonic agents. Also, the accuracy of both increases as more data is obtained, however the Fourier domain approach requires the data to be recorded over many cardiac cycles while the segmentation approach can be computed from as little as 1 cardiac cycle. The Fourier domain approach also effectively linearizes the cardiac waveform data, resulting in a smaller measure of the average change in volume over the cardiac cycle. Conversely, the segmentation approach is more susceptible to noise, though by averaging over many heart beats this effect is minimized. Littleton et al, 2012 showed that cardiac output decreases with increasing cholesterol, and that there is a significant difference in CO between 0.1 CH in the diet compared to 8 CH in the diet. This data was utilized to compare the manual measurement of CO from Littleton et al, 2012 to our automated methods (Figure S2). CO was analyzed with both the Fourier and segmentation approaches and compared to manual analysis. As in manual measurements, both automated methods detected a significant difference between the lowest treatment group (0.1 CH) and the highest treatments group (8 CH). When compared to the manual methodology, the segmentation approach lead to a similar correlation between CH and CO as indicated by analogous R2 values, as well as similar slopes (Figure S2. This demonstrates congruous strength of detected effect of CH on CO and similar sensitivity in the analysis paradigms. The Fourier approach shows slightly decreased R2 and slope value. The error in the measurements in the Fourier analysis is however less than both manual and segmentation approaches. Longer-term dietary intervention in zebrafish in a previous study [18] indicated that when ground hawthorn leaves and Pentagastrin web flowers were added to food combined with cholesterol there was an interaction effect between hawthorn and cholesterol to improve cardiac output compared to cholesterol treated fish. In this study, treatment with MHE lead to a statistically significant MedChemExpress Salmon calcitonin increase 
in SV and EF. These results therefore agree with one another, as our method in this present endeavor required that we introduce hawthorn in an egg yolk and water solution. Also, the approximately 20 increase in SV and EF due to MHE treatmentsuggests it to be a clinically relevant inotropic agent, supporting its present use as a cardiotonic in heart failure [26]. Previous studies on alcoholic extracts of hawthorn leaves and flowers demonstrate increased cardiomyocyte contractility and vasodilatory effects in cell culture and ex vivo experimental paradigms [26]. It is therefore likely that the increase we detected in SV and EF is due to a combination of vasodilation and increased cardiac inotropy, combining to improve cardiac function. In this study we have provided a simple and robust platform for testing the efficacy and side.And trough of each wave in a dataset and subtracting the mean maximum from the mean minimum). Two different datasets were analyzed in these measurements–one from a healthy heart and one from an erratically beating heart (Figure S1). The results demonstrate that in both cases, the segmentation approach based on frequency- and time-domain analysis better predicts manual measurements. While the methods yield different absolute values of SV, their ability to detect changes in these parameters is nearly identical (figure 5 and 5c). These methods of analysis therefore verify one another in their ability to detect the effects of cardiotonic agents. Also, the accuracy of both increases as more data is obtained, however the Fourier domain approach requires the data to be recorded over many cardiac cycles while the segmentation approach can be computed from as little as 1 cardiac cycle. The Fourier domain approach also effectively linearizes the cardiac waveform data, resulting in a smaller measure of the average change in volume over the cardiac cycle. Conversely, the segmentation approach is more susceptible to noise, though by averaging over many heart beats this effect is minimized. Littleton et al, 2012 showed that cardiac output decreases with increasing cholesterol, and that there is a significant difference in CO between 0.1 CH in the diet compared to 8 CH in the diet. This data was utilized to compare the manual measurement of CO from Littleton et al, 2012 to our automated methods (Figure S2). CO was analyzed with both the Fourier and segmentation approaches and compared to manual analysis. As in manual measurements, both automated methods detected a significant difference between the lowest treatment group (0.1 CH) and the highest treatments group (8 CH). When compared to the manual methodology, the segmentation approach lead to a similar correlation between CH and CO as indicated by analogous R2 values, as well as similar slopes (Figure S2. This demonstrates congruous strength of detected effect of CH on CO and similar sensitivity in the analysis paradigms. The Fourier approach shows slightly decreased R2 and slope value. The error in the measurements in the Fourier analysis is however less than both manual and segmentation approaches. Longer-term dietary intervention in zebrafish in a previous study [18] indicated that when ground hawthorn leaves and flowers were added to food combined with cholesterol there was an interaction effect between hawthorn and cholesterol to improve cardiac output compared to cholesterol treated fish. In this study, treatment with MHE lead to a statistically significant increase in SV and EF. These results therefore agree with one another, as our method in this present endeavor required that we introduce hawthorn in an egg yolk and water solution. Also, the approximately 20 increase in SV and EF due to MHE treatmentsuggests it to be a clinically relevant inotropic agent, supporting its present use as a cardiotonic in heart failure [26]. Previous studies on alcoholic extracts of hawthorn leaves and flowers demonstrate increased cardiomyocyte contractility and vasodilatory effects in cell culture and ex vivo experimental paradigms [26]. It is therefore likely that the increase we detected in SV and EF is due to a combination of vasodilation and increased cardiac inotropy, combining to improve cardiac function. In this study we have provided a simple and robust platform for testing the efficacy and side.