220 C and 250 C, respectively. 2.four. Identification on the Important oil Constituents The important oils obtained in the fresh sage plus the dried batches had been identified depending on the experimental retention index (RI) calculated with references to regular n-alkenes series (C8 40 ), plus the retention indices reported inside the literature beneath related GC experimental situations. Moreover, the identification with the compounds was carried out determined by their retention time and comparisons with the mass spectral libraries (National Institute of Requirements and Technologies (NIST-11) and Wiley Database). The relative percentages of the constituents have been calculated in the location under the peak obtained in the GC-FID chromatogram. 2.5. In Vivo Hepatoprotective Assay two.five.1. Experimental Animals The in vivo hepatoprotective MT2 Accession activity of the sage critical oil batches was performed working with Wistar male rats weighing about 20050 g, which were kindly provided by the animal property facility of your College of Pharmacy, Qassim University. The rats had been housed in appropriate humidity and temperature (25 2 C) and given a normal diet and water ad libitum. The animals had been kept in a pathogen-controlled, air-conditioned space inside the animal house. Each of the experiments have been performed, in line with the guidelines for animal studies that had been approved by the Ethical Committee of College of Pharmacy, Qassim University, KSA. 2.5.2. Acute Toxicity Research Briefly, ten-weeks-old male Wistar rats (n = 15), weighing 20050 g, getting overnight fasted, have been weighed, as well as a single dose of 50, 100, and 200 mg/kg (n = 5/group) of Salvia ROCK2 drug officinalis vital oil was administered, using the oral route. The animals have been observed for abnormality in behavior and movements for the first 3 days and mortality for up to two weeks. According to the outcomes, 20 mg/kg, ten on the maximum administered dose based on the Hedge and Sterner scale, was selected for evaluation of the hepatoprotective activity [36]. 2.5.three. Animal Groups A total of 40 ten-week-old Wistar male rats, weighing 20050 g, have been divided randomly into eight equal groups (n = five); the very first group was considered the handle and received oral supplementation of saline, using an orogastric cannula. The second group ofMolecules 2021, 26,5 ofanimals (negative control) received oral administration of AAP (inside a dose of 500 mg/kg) once day-to-day starting from the 11th day of the experiment for 5 consecutive days to induce liver injury. The third, fourth, fifth, sixth, and seventh groups of animals received 20 mg/kg BW (bodyweight), once daily, for 15 days the crucial oils obtained in the fresh herb (FH), one-week (1WDH), two-week (2WDH), three-week (3WDH), and four-week dried herb (4WDH) from the Salvia officinalis, respectively. The animal groups (third to seventh groups) received AAP to induce liver injury (in a dose of 500 mg/kg) beginning in the 11th day from the experiment for five days. Typical liver support was administrated to group quantity eight, which was pretreated with silymarin (oral dose: 100 mg/kg, 15 days), and AAP for the final five days. At the end of the experiment, blood samples were collected by retro-orbital puncture, serum was separated from all groups’ collected blood for the determination of liver functions (ALP, AST, ALT, and total protein) also as kidneys functions (urea and creatinine) and also the lipid profile (triglycerides and total cholesterol) analyses. two.5.four. Determination of Liver, Kidneys Functions, and Lip
