Ge), specially created to separate urine and faeces andS. no. 1 2 three 4 5 groups Handle (ten ml/Kg b. wt) Common (Frusemide 10 mg/kg b.wt) Alcoholic extract of roots of C.pareira Low (100 mg/kg b.wt) Alcoholic extract of roots of C.pareira Medium (200 mg/kg b.wt) Alcoholic extract of roots of C.pareira High (400 mg/kg b.wt)DISCUSSIONMedicinal plants and Cathepsin D Protein Gene ID botanicals offer a natural safeguard against ailments and are a substantial remedy for specific illnesses. Diuretics have proved to be particularly valuable in the treatment of mild to moderate hypertension and also in enhancing the impact of other antihypertensive agents. Diuretics relieve pulmonary congestion and peripheral oedema. These agents are useful in lowering volume over load and relieve orthopnea and paroxysmal nocturnal dyspnoea [19] in CCF and acute left ventricular failure. They decrease plasma volume and subsequently venous return to the heart. This decreases the cardiac function load, oxygen demand and plasma volume and also decreases blood pressure. Thusna+ mmol/l 113.03 + 2.16 191.05+2.09 129.40+2. total urine Vol (ml/kg b.wt/5 h) 13.45?.02 22.23?.01 15.20?. K+ mmol/l 51.09 + 1.51 87.81+1.60 64.13+1. Cl- mmol/l 82.95 + 1.42 129.06+1.67 94.42 + 1.73 109.44+1.20 121.39+2.0017.41?.02 20.46?.164.99+2.00 184.53+2.77.93+2.67 85.11+1.[Table/Fig-1]: Impact of alcoholic extract of roots of Cissampelos pareira on urine volume and electrolyte concentration in hydrated rat model in albino rats Values expressed as mean ?S.E.M.,n=6, Significance at p0.05, p0.01, p0.001, Compared with control group (One particular Way ANOVA followed by TL1A/TNFSF15 Protein Formulation Dunnetts `t’ test).Journal of Clinical and Diagnostic Analysis. 2014 Might, Vol-8(five): HC01-HCjcdr.netSuresh Babu Sayana et al., Evaluation of Diuretic Activity of Alcoholic Extract of Roots of Cissampelos Pareira in Albino Ratssaponins, organic acids [1,17], steroids, carbohydrates, tannins, phenolic compounds, terpenoids [22], alkaloids [23], glycosides [24], sterols [25], sesquiterpenes aminoacids, carotinoids [26] in distinctive plant extracts. Alcoholic extract of roots of Cissampelos pareira was identified with most of these plant phytochemical substances described above. Hence it might be reported that the observed diuretic activity is as a consequence of these above phytoconstituents.CONCLUSIONResults showed that single dose administration of alcoholic extract of roots of Cissampelos pareira as 100,200 and 400 mg/Kg and regular Furosemide (10 mg/kg) have increased the urinary output in addition to an increase in concentration of Sodium, Potassium and Chloride ions in urine. Alcoholic extract of roots of Cissampelos pareira mg/Kg developed a greater diuretic activity which can be comparable to that of common Furosemide (ten mg/kg). In regular medicine the plant is used for its diuretic activity. Ours scientific study come up with identification of a lot of phytoconstituents reported earlier for this diuretic impact in our alcoholic extract of roots of Cissampelos pareira. Hence our study supports and justifies the rationale behind the folklore use of roots of C.pareira for its diuretic activity.[Table/Fig-2]: Impact of alcoholic extract of roots of c. pareira on urinary sodium, pot assium, chloride (mmol/l) ions concentration in hydrated rat model in albino ratsACkNOwLEDGEMENTSThe authors are very much grateful to Dr. S.M.Shanth Kumar Ex Principal, V.L. College of Pharmacy and Dr. V.Hemanth Kumar Principal V.L. College of Pharmacy, Raichur, Karnataka for offering the laboratory facil.
