2350) was employed. The chromatographic analysis of KM-408 was performed on a Fusion RP-18 column (150 mm 4.six mm; particle size 4 ) from Phenomenex with isocratic elution working with a mobile phase composed of ammonium acetate (0.15 M, pH 4.4) and methanol (45:55, v/v) at a flow price of 1.1 mL min-1 at 25 . The injection volume was 20 . Based on the registered absorption spectrum detection wavelength was chosen as 230 nm. The evaluation time was 15 min. Irradiation circumstances. Irradiation was conducted in a climatic chamber KBF-ICH 240 APT.lineTM (Binder GmbH, Tuttlingen, Germany) at 25 and 60 humidity applying UVA radiation (32000 nm) with maximum emission at 365 nm. The intensity of radiation was determined utilizing a radiometer form VLX-3 W, Vilber Lourmat, using a CX-365 sensor, to become each and every time of 0.25 mW m-2. The distance amongst the samples plus the radiation source was 13 cm. Degradation in acidic option. For acidic degradation, about one hundred mg of KM-408 was accurately weighed, transferred to a volumetric flask of 10 mL capacity and dissolved in 0.5 M hydrochloric acid. 8 mL of your examined answer was transferred to a glass vial and heated in an oven at 70 . The sample volume of 0.five mL was taken and diluted with 0.five mL of water. The remedy was analyzed three times by HPLC system. Degradation in fundamental solution. For basic degradation, one hundred mg of KM-408 was accurately weighed, transferred to a volumetric flask of 10 mL capacity and dissolved in 0.five M NaOH. 4 mL of examined remedy was transferred to glass vials of eight mL capacity and heated in an oven at 70 . The volume of 0.5 mL was taken and diluted with 0.five mL of 0.5 M HCl. The resolution was analyzed three instances by HPLC strategy. Degradation in phosphate buffer at pH = 7.0. 100 mg of KM-408 was weighed, transferred to a volumetric flaskA. Waszkielewicz et al.of 10 mL capacity and dissolved in phosphate buffer at pH = 7.0. The volume of 8 mL of examined answer was transferred to glass vials of eight mL capacity and stored in a dark spot at 25 or heated in an oven at 70 . The volume of 0.five mL of solution was taken and diluted with 0.5 mL of water. The solution was analyzed by HPLC strategy. Degradation in the presence of oxidative agent. 100 mg of KM-408 was weighed, transferred to a volumetric flask of ten mL capacity and filled up to a volume with 1.five H2O2. The samples were stored at 25 . The volume of 0.5 mL of solution was taken and diluted with 0.5 mL of water. The remedy was analyzed 3 occasions by HPLC method.TIM Protein MedChemExpress 100 mg of KM-408 was weighed, transferred to a volumetric flask of 10 mL capacity and filled as much as a volume with 0.IL-11 Protein custom synthesis 001 M CuSO4.PMID:24360118 The volume of 8 mL of examined resolution was transferred to glass vials of eight mL capacity and heated in an oven at 40 . The volume of 0.five mL of solution was taken and diluted with 0.five mL of water. The resolution was analyzed by HPLC method. Degradation within the presence of reducing agent. 100 mg of KM-408 was weighed, transferred to a volumetric flask of 10 mL capacity and filled as much as a volume with 0.005 M Na2S2O3. eight mL from the examined option was transferred to eight mL glass vials and stored at 25 and 40 . The volume of 0.five mL of option was taken and diluted with 0.five mL of water. The option was analyzed 3 times by HPLC process. Photodegradation below UV irradiation. 25 mg of KM408 was weighed, transferred to a volumetric flask of 25 mL capacity and filled as much as a volume with methanol. two mL of your examined option was transferred to a quartz Petr.
