Actually Intriguing New Gene finger variants of zinc finger motifs act as ubiquitin E3-ligases and concentrate on proteins like p53 to the proteasome. Given that PHD and RING finger motifs are the two types of zinc fingers, it was speculated that some PHDs also act as ubiquitin E3-ligases, but closer inspection of PHD locations did not confirm this hypothesis. Dependent on this background, and a preceding research indicating that INGs bodily interact with at the very least sixteen proteins immediately 1621523-07-6 concerned with proteasomal degradation these kinds of as regulatory subunits of equally the 20S and 26S-proteasome, we asked a) no matter whether ING1 stabilizes p53, and if so, b) whether ING might do this through impacting ubiquitin metabolic rate, thus shielding p53 from proteasomal degradation. We discovered a region adjacent to the PHD of ING1 that acts as a ubiquitin-binding area. We also discovered that ubiquitin competes with PI signaling lipids for ING1 binding and that physiological BAY-1841788 stages of ING1 stabilize monoubiquitinated types of the p53 tumor suppressor by means of its UBD.
