Product Name :
Nitric Oxide (total), detection kit
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Store all components at 4°C, except nitrate reductase at -20°C.
Description:
Sensitive, efficient, convenient method for detection of total Nitric oxide in just 40min Efficient – only 50 µl diluted sample needed, saving your precious sampleSensitive – detect as little as 0.{{2230186-18-0} site|{2230186-18-0} Purity & Documentation|{2230186-18-0} Data Sheet|{2230186-18-0} manufacturer} 625 µmol/LTrusted – widely cited in peer-reviewed publicationsFast – results from up to 80 samples in duplicate in just 40 minutes The Nitric Oxide (total), detection kit is based on the enzymatic conversion of nitrate to nitrite by the enzyme nitrate reductase, followed by the Griess reaction to form a colored azo dye product. Quantification is performed by measuring absorption at 540-570 nm. Only 0.050 ml diluted sample needed per well saves precious sample.Nitric oxide (NO) is a major mammalian secretory product that initiates host defense, homeostatic and developmental functions by either direct effect or intercellular signaling. NO is the product of a five-electron oxidation of the amino acid L-arginine mediated by nitric oxide synthase.{{1352066-68-2} MedChemExpress|{1352066-68-2} Protocol|{1352066-68-2} Data Sheet|{1352066-68-2} manufacturer} As a direct effector, NO is thought to activate regulatory proteins, kinases and proteases that are directed by reactive oxygen intermediates.PMID:27057822 Activation of the immune system is associated with an increase in macrophage NO production. Soluble NO exerts a variety of homeostatic influences as an activator of soluble guanylyl cyclase, a neuronal potentiator, a peripheral nervous system neurotransmitter, and a contraction regulator of both smooth muscle and vascular tissue. In addition, NO has been linked to the formation of olfactory and synaptic memories and remodeling. Nitric Oxide (total), detection kit Kit graph Nitric Oxide (total), detection kit Kit graph
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Additional Information :
| Application Colorimetric detection | Application Notes For the quantitative determination of total Nitric Oxide in culture supernatants, plasma, serum, saliva, and urine from any species. Cited sample type includes tissue (ref). | Contents Microtiter plate, Nitrate Reductase, Nitrate Reductase Storage Buffer, NADH, Nitrate Standard, Reaction buffer concentrate, Griess Reagent I, Griess Reagent II | Sensitivity 0.625 µmol/L (range 3.125 – 100 µmol/L) | Species Reactivity Species independent
